Melvin's digital garden

Lipidomics

CREATED: 200612290616 Related: http://www.lipidprofiles.com/main.htm

** Metabolics

lipidomics

head group

tails

sugars

toxins

** Uses

energy store (metabolism)

cellular membranes

foreign lipids - diseases caused by bacteria

signaling

** Interactions

lipid receptors

interfacial bindings

integral interactions

with proteins

** Equipment

ESI-MS

Tandem MS

Precursor Ion Scan (class specific fragmentation)

Thin layer chromatography

** Examples

growing vs dormant bacteria

difference in lipids

look at lipid profile of different states, overlay difference profile

identification of ions in difference profile

identification of biochemical pathway

** Analysis of lipid profile

Correlation Optimized Warping algorithm

alignment of profile to eliminate warping

Identification of the peaks

Similarity of lipids (molecular mimicry)

** Application of lipidomics

identification of biochemical pathways

novel lipids

as a test for diseases

eg. TB by testing for lipids not found in human

blood lipids as biomarkers

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