Genomic sources of regulatory evolution

CREATED: 200810200259 Speaker: Patricia Wittkopp

• cis vs trans regulation, inter vs intra species sites ** cis regulation (binding site): allele specific ** trans regulation (transcription factor): affects both alleles
• distinguishing cis vs trans regulatory changes using F1 heterozygous and pyrosequencing to determine #transcripts from each allele
• contribution of cis/trans between D. Melanogaster and D. Simulans ** interspecies more cis regulatory difference ** use 2 inter comparisons to derive intraspecies difference
• Model of neutral evolution doesn’t explain the difference
• Hypothesis 1 ** trans regulatory maintained by mutation-selection balance ** mutational target is larger in trans
• Hypothesis 2 ** cis regulatory are fixed preferentially ** positive selection for cis or negative selection against trans
• Properties of regulatory changes (mutation) based on yeast cells using FAS (Florescent assistant sorting)