Fernandez2005

CREATED: 200906170314 LINK: url:~/Modules/Literature/Fernandez2005.pdf

Shows how FTIR can be used for histopathologic recognition

FTIR permits high-throughput and spatially resolved measurements but lack sensitivity for complex determinations (ref 14) and are limited to detecting either significant biochemical changes (ref 15) or foreign inclusions (ref 16)

Use of FTIR typically involves the following steps

measurement of large numbers of spectral profiles from every tissue subtype

identification of relevant spectral markers

organization of markers into a prediction algorithm

statistical validation of the automated process

Histologic classes contain identical chemical compoenents, their characteristic spectra are similar but reveal small differences in specific absorbance features. Univariate analyses are unable to distinguish all histologic constituents.

Subtle molecular structural changes are indicated by peak shifts, band shapes and relative intensity changes. Example of spectral features: ratio of absorbance at 1080cm^-1 (phosphodiester) to 1545cm^-1 (amide II) provides a definitive metric for discriminating epithelial cells from stromal cells.

Relate a small set of hand crafted spectral metrics (with physical significance to tissue biochemistry) to specific histologic entities. Different from typical practice where large spectral regions are reduced to smaller sets of variables by mathematical means. Set of 18 metrics provided accurate segmentation.