Applying the neutral indel model across metazoan genomes

CREATED: 200810200457 Speaker: Chris Ponting

Gerton Lunter’s indel model

• consider length of inter-gap segments, under netural model should follow geometric distribution
• at most 0.09% of all AR are under selection

Overrepresentation of long inter-gap distances

• purifying selection removes delerious indels
• 1.56-2.25% of human genome are functional but non-coding

How much protein coding regions?

• compare against mouse genomes
• about 1.06%

Are non coding transcripts functional?

• many of the 3122 nc transcripts exhibit purifying selection

NIM work well for many paris, except for closely related primates

• due to PolyA microsatellites in ALUs at 150bp apart
• departure at low distances probably due to errors in sequencing/assembly

10% of human genome appears to be constrainted by indel purifying selection

• ref: The functional repertoires of metazoan genomes, Nature Review Genetics
• much turn over in functional sequence, functional but not conserved!